from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). #ID3-STD) L-Tryptophan (Sigma cat. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 6 L (with SpectraMax i3x System) Title: SpectraMax MiniMax 300 Imaging Cytometer | Molecular. g. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 0 OD Photometric resolution: 0. The Absorbance 96 is a uniquely designed microplate reader for assays in 96-well format. ” Promega Corp. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. With optimized reagents, option to operate the. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Microplate method: Pipet 150 µL aliquots of each sample, standard and blank into the designated wells of the microplate. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. In addition, the iD5 reader measures TRF and FP and. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. 384 Awareness Technology ChroMate BioTek Synergy Mx. 2 W x 44. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The SpectraMax Plus 384 Microplate Reader is a good choice for running red wine phenolics assay described by Heredia et al. Make sure to Data 96孔微孔板的数据对比. Plate wash time (300 µl). MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aThe Molecular Devices Aquamax 4000 With 96 Well Head AQUAMAX 4096-G is a quality addition to the Molecular Devices lineup. It also integrates with the. The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. Five-second read per plate. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 39. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Pick the best speed, sensitivity, and price for your needs. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Highly customizable low volume applications Molecular Devices unique SpectraDrop Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. University of Reading Simon Lydford: Molecular Devices (UK) Ltd. i3x. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 2 OD at 200 nm. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 012 fmol 0. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR. 40266. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Visible absorbance -. The assay kit is optimized for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. We would like to show you a description here but the site won’t allow us. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. #3610) BrightMax sealing films (Genesee cat. Integrated stacker option. It features a modular design that allows users to add or change detection cartridges according to their needs. SOFTmax PRO allows you to select excitation and emission values from the literature and simply input their values for the monochromator settings. Microplate Readers. 300 µL of Detergent Compatible Bradford reagent was added to each well. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. SpectraMax® M3/M4/M5/M5e, GeminiTM EM/XPS, FlexStation® 3. Both readers were heated to 37ºC prior to setting up . Related Products: Low Binding Plates. We have been using this for DNA measurements 260/280 nm, colorimetric assay measurements like MTT assays, and ELISAs. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. #PLUS 384) Methods. Molecular Devices gmbH Tel. 03 pM 384-well: 0. 7°C and the bottom to 36. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. Formats available are strip washers, full plate washers and combination washer-dispensers. “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. Running both standard spectrophotometer and microplate. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. GxP regulated industry assessments of microplate readers. 05. /well integration in 96-well luminescence mode and 0. Microplate luminescent reader assay for ERK activity. Outer wells are aligned for proper viewing with FLIPR. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0. LVF Monochromator™ : Excitation and Emission. in space. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Setup Guide. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. 8 Pages. 05. 0200-6262. It is a UV/Vis spectrophotometer/microwell reader with built-in cuvette port and microplate drawer. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. Omnia® Compatible Microplate Reader Documentation Version No. , Sunnyvale, CA 94089. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. 92: 88. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. 50 racks/case for 384-well (16 channel pipettor head. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Three low volume 24-well bottom slides. 8. For more sample throughput, the system can be. Molecular Devices SpectraMax 384 Plus Microplate Reader. A single-mode plate reader is a good starting point. Brand: MOLECULAR DEVICES. Supplementary Information. High. Key applications: ELISA, nucleic acid, endotoxin testing. All stand-alone instrument functions can be controlled using the software. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. We demonstrate that miniaturizing the assay in 96-well half area microplates, and using the flexible liquid transfer features of the FlexStation ® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values for agonists and IC 50 values for antagonists. 00 Add to cart; Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. (96, 384, or 1536 wells) and the "Select Specific". Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. To confirm the accurate positioning of arrayed colonies, we performed an absorbance scan in a 96-well scheme with 30 × 30 scans per well for an OmniTray agar plate with 96. 14: Average %CV. Built in near field communication (NFC). 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Transfer of an assay to 96-well microplate technology increases throughput significantly. ***Figure 1. Endpoint, kinetic, spectrum and area-well scanning Click here. All stand-alone instrument functions can be controlled using the software. 3. Easy-to-use, intuitive, configurable microplate readers with industry-leading, SoftMax Pro microplate software solution for increased productivity. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Figure 2. 030 fmol Fluorescence Polarization (FP). This reader provides visible. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. for ValitaTiter Plus standards read on Molecular Devices readers. Create custom fluorescence assays without having to purchase expensive filter sets. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ EMax Plus R2 = 0. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Efficient cell washing with minimal cell loss and optimal assay performance. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. 0 96 Well Plate. Four wash/rinse bottles are included and configurable for both 96- and 384. The SpectraMax® ABS Plus Microplate Reader can run . SpectraMax 250 User. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. (Figure 1). Microplate readers use a vertical light path so the distance of the. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 5 C @ 37 C well-to-well Computer Specifications Processor Intel I5-4300U CPU Ram 16. Compare plate readersThe Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. book Page 9 Wednesday, July 7, 2010 2:09 PM. 96-well: 0. fluorescence microplate reader from Molecular Devices. Flexible Microplate Format The GEMINI system allows you to select from 6-, 12-, 24-, 48-, 96- and 384-well microplate formats for your fluorometric measurements. $489. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. Growth medium was then replaced with fatty acid uptake solution. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. 5 mm. F3/F5 Multi-Mode Microplate ReadersBulk Kit (10-plate size, Molecular Devices P/N R8347) HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesThe SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Or scan up to 384 wells at any time to determine the best assay conditions. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. We would like to show you a description here but the site won’t allow us. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. A 1:10 serial dilution of ATP was prepared in medium. Consult Molecular Devices's entire Threshold Immunoassay System catalogue on MedicalExpo. Multimode Reader Comparison Chart. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Integrated stacker option. 1536-well: 0. In . SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Seller assumes all responsibility for this listing. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Description. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Fluorescence Microplate Readers. Bioz Stars score: 86/100, based on 1 PubMed citations. Microplate readers can be either single-function instruments or “multimode. Fluorescence Microplate Reader Comparison Chart. Bioz Stars score: 86/100, based on 1 PubMed citations. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Abstract and Figures. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Samples were run in triplicate. M3-M5e_manual. microplates or in a cuvette at a selected. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). 384 well polystyrene microplates have plate dimensions of 127. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well3. This reader also makes use of a unique temperature-independent methodology for path length correction. Four wash/rinse bottles are included and configurable for both 96- and 384. Page 11: Reader ComponentsBioTek Synergy HTX Microplate Reader - Absorbance Only Specifications. Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. These instruments are all elegant and simple in their form and function. The EMax® Plus Microplate Reader is designed to be a versatile and robust microplate reader for research laboratories. adapter plate in drawer of Plate Reader with well A1 in position A1 of the drawer. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPlus Microplate Reader, the R2 value was 0. An industry-renowned instrument for monitoring GPCRs and ion channels. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. 3. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are formed in single well constructs 12. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. The system is operated through the industry-leading SoftMax® Pro. Endpoint, kinetic, spectrum. The Perkin Elmer Wallac 1420. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 USA Tel: 408. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Reading Capabilities: cuvette or microplate. SpectraMax® 340PC384 Microplate Reader for. About;. 020 fmol 0. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. These robust readers have been placed in labs from. 1-color 96-well . SpectraMax® Microplate Reader Suite of Products Knowledge Base. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Taking into account the price differences between each seller and each country. The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. the iD5 reader’s filter system. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. The SpectraDrop Microplate’s slide design eliminates the need for calibration and gives consistent well-to-well reads with CVs below 5%. 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability. M3-M5e_manual. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. Reading a Microplate . Materials. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Incubate the plate at 37 °C for approximately one hour. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. Final BSA Concentration (µg/ml) in assay BSA Volume NanoOrange. Temperature Control: Ambient +5C up to 42C. Accuris MR9600 SmartReader 96 Microplate Absorbance Reader. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. Published Dec 16, 2020 | Updated Dec 16, 2020. 0. 1 FP • Fluorescence Polarization detection for fluorescein- or . The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. This unlocks the transport slide. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. For more sample throughput, the system canRating: 3. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Align the two slots on the bottle holder with the two pegs on the rail. Molecular Devices M5 Plate Reader Plate Calibration Form . # 651101) the iD5 reader’s filter system. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. With various on-board features and capabilities,. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. in a 96-well microplate. A control well was set up to verify. Listing: #4772697. Plate Formats: 6 to 384-well microplates. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. Condition: Used - Good. g. Step 1: Turn on your microplate reader. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Add 150 µL reagent to each well. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. The VersaMax reads 96-well microplates. Please refer to the Obsolete Microplate Reader Models for more information. Deep well blocks available in 24, 96 and 384-well formats Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Molecular Devices' microplate readers. It also integrates with the powerful SoftMax Pro Software for. Learn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. For biochemical. M5. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Flexible for 6-well to 384-well microplates and cuvettes. Blank wells containing 50 µL culture medium only (n = 8) were also prepared. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Please click on the links below to view the Microplate Reader Comparison Tables. the appropriate well of the microplate. absorbance, fluorescence, and luminescence with the added functionality of. Affordable multi-mode microplate reader with . keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The system provides automation for up to 50 microplates for easy walkaway automation. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. i3x. #N207) LAL Reagent Water (Lonza cat. 585The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Vrla used the device to quantify the concentration of a small molecule produced by. The validation kit included individual tubes of diluted fluorophores:. #G7294). Retrieving. 0200-6263. 0 OD at. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Vmax Kinetic Microplate Reader with SOFTmax® Pro from Molecular Devices, now part of MDS Analytical Technologies. 96- or 384-well microplate compatibility. Eight filter modes enable applications such as protein quantification, cell viability and ELISA. 5°C at 37°C. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . book Page 1 Wednesday, October 12, 2011 4:56 PMBuilt around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. 0 fmol/well FITC 200 µL in 96 wells. 2 2600A ValitaTiter. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. com. Keywords: fluorescence polarization, trf, fret, igg. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. SOP Title: Use and. AquaMax 4000 Microplate Washer (Molecular Devices Cat. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0 500 1000 1500 2000 2500 3000 3500 time (sec. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. Published Dec 16, 2020 | Updated Dec 16, 2020. The color touch screen provides a visual user interface, making programming fast and intuitive. DNA concentration (ng/mL) Average RFU. For more sample throughput, both readers can be easily integrated into full roboticThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 4. ScanLater Western Blot Detection System. Both COC microplates, the µCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Protein quantitation Cross-talk < 0. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Description. Never order or replace filters to optimize an assay again!microplate readers from Molecular Devices. Equipped with scanning, shaking and kinetics modules. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. and luminescence microplate reader validation DATA SHEET. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader.